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- Optimization of light-inducible Gal4/UAS gene expression system in mammalian cells
Sep 17, 2020
Optimization of light-inducible Gal4/UAS gene expression system in mammalian cells
26th August, 2020
Light-inducible gene expression systems represent powerful methods for studying the functional roles of dynamic gene expression. Here, we developed an optimized light-inducible Gal4/UAS gene expression system for mammalian cells. We designed photoactivatable (PA)-Gal4 transcriptional activators based on the concept of split transcription factors, in which light-dependent interactions between Cry2-CIB1 PA-protein interaction modules can reconstitute a split Gal4 DNA binding domain and p65 transcription activation domain. We developed a set of PA-Gal4 transcriptional activators (PA-Gal4cc) which differ in terms of induced gene expression levels following pulsed or prolonged light exposure, and which have different activation/deactivation kinetics. These systems offer optogenetic tools for the precise manipulation of gene expression at fine spatiotemporal resolution in mammalian cells.
Yamada, M., Nagasaki, C.S., Suzuki, Y., Hirano, Y. and *Imayoshi, I. (2020) Optimization of light-inducible Gal4/UAS gene expression system in mammalian cells. iScience, 23, 101506, September 25, 2020.
https://doi.org/10.1016/j.isci.2020.101506
